Effects of limbs ischemic postconditioning on mitochondria structure and function after cerebral ischemic reperfusion in diabetic rats
- VernacularTitle:肢体缺血后处理对糖尿病大鼠脑缺血再灌注损伤线粒体结构和功能的影响
- Author:
Yan LI
;
Cuilan WANG
;
Yingyun LU
- Publication Type:Journal Article
- Keywords:
limbs ischemic postconditioning;
reperfusion injury;
diabetes mellitus;
mitochondria
- From:
Journal of Clinical Neurology
1992;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate effects of limbs ischemic postconditioning induced remote postconditioning (RPostC) on mitochondria structure and function after focal cerebral ischemic reperfusion(I/R) injury in diabetic rats. Methods The diabetic rats models were induced by injecting streptozotocin into abdominal cavity. I/R rats models were made by MCAO with thread. Forty male SD rats models were assigned to 4 groups: control group; sham operation group; I/R group;RPostC group ( n=10 ) . 6 h after the reperfusion, the brains were obtained for HE staining. The mitchondria were isolated and content of MDA,the activities of SOD,Na+/K+-ATPase,Ca2+-ATPase and GSH-Px were tested. Morphological changes of neuronal mitochondria were observed by electronic microscope. Results In the I/R group,the content of mitchondria MDA[(4.99?1.25) nmol/mgprot]were markedly increased (P
