Construction of humulus scandens pollen major allergen pcDNA3.1-LC2 DNA vaccine and study of its immunogenicity in mice
- VernacularTitle:葎草花粉主要变应原核酸疫苗pcDNA3.1-LC2的构建及其免疫原性鉴定
- Author:
Yonghong ZHANG
;
Xiuzhen SUN
;
Yun LIU
;
Jing XU
- Publication Type:Journal Article
- Keywords:
asthma;
humulus scandens grass pollen allergen;
DNA vaccine
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
2010;31(1):51-53,78
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct humulus scandens pollen major allergen DNA vaccine pcDNA-LC2 and evaluate its immunogenicity. Methods Humulus scandens pollen allergen gene was digested from recombinant plasmid pTripIEx2-LC2 by EcoRⅠ and HindⅢ of restriction endonuclease, and then inserted into expression vector pcDNA3.1(-). A large amount of endotoxin pcDNA3.1-LC2 purified plasmid was extracted after successful construction, and animal experiment was carried out to study its immunocompetence. Results Sequencing results showed that 672bp in the coding sequence of frames of recombinant pcDNA3.1-LC2 was in line with the original base line, without deletion or mutation. pcDNA3.1-LC2 of pollen allergen humulus scandens allergen vaccinated BALB/c mice. Double immunodiffusion test showed that pcDNA3.1-LC2 could be expressed and produce antibodies in mice. Conclusion Humulus scandens grass pollen major allergen DNA vaccine pcDNA3.1-LC2 has been successfully constructed. It can be expressed in mice, produces humulus scandens grass pollen allergen specific antibody and has a good immunogenicity.
