Effect and its mechanism of cyclin dependent protein kinase inhibitor treatment on experimental herpes simplex encephalitis
- VernacularTitle:细胞周期素依赖性蛋白激酶抑制剂治疗实验性单纯疱疹性脑炎的效果及其机制
- Author:
Yu ZHOU
;
Yuanjin GUO
;
Yuanwu MEI
- Publication Type:Journal Article
- Keywords:
herpes simplex encephalitis;
cyclin dependent protein kinase;
microglia;
apoptosis
- From:
Journal of Clinical Neurology
1992;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect and its mechanism of cyclin dependent protein kinase inhibitor Olomoucine treatment on experimental herpes simplex encephalitis(HSE). Methods 45 mice were randomly assigned to HSV-1 sham-infected group (n=15), HSV-1 infected control group (n=15) and Olomoucine treated group (n=15). Flow cytometry was used to detect the expression of brain cells surface antigen CD11b. The reverse transcription polymerase chain reaction (RT-PCR) to detect the expression of tumor necrosis factor-? (TNF-?) and Interleukin-6 (IL-6). TUNEL method was adopted to detect neural cells apoptosis. Pathologic slices of brain tissues were used to identify tissue morphology differences. The survival rates were compared between the treatment groups Acyclovir (n=20) and Olomoucine (n=20) by the method of Kaplan-Meier 2 weeks after HSV-1 infection. Results After HSV-1 infection, the expression of brain tissue CD11b, TNF-?, IL-6 were significantly increased compared with sham-infected group (allP
