The experimental study of the influence of FN-TPO gene modified mesenchymal stem cells on cord-blood hematopoietic stem cell engraftment
- VernacularTitle:FN-TPO基因修饰的骨髓间充质干细胞支持脐血造血干细胞植入的实验研究
- Author:
Lei ZHANG
;
Jie YU
;
Shu MO
- Publication Type:Journal Article
- Keywords:
Mesenchymal stem cells;
Hematopoietic stem cell;
Fibronectin;
Thrombopoietin;
Engraftment
- From:
Chinese Journal of Blood Transfusion
1988;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the influence of Fibronectin-Thrombopoietin(FN-TPO) gene modified human bone marrow mesenchymal stem cells(MSCs) on the engraftment of cord blood hematopoietic stem cells.Methods FN-TPO gene modified human bone marrow MSCs combined with cord blood mononuclear cells(CB-MNC) were transplanted to sublethal dose treated severe combined immunodeficiency disease(SCID) mice.After transplantation,these mice were observed for 4 weeks.Peripheral blood cell counts were performed at different time point to assay the hematopoietic system status of the mice.Four weeks after the transplantation,human-sourced cell integration was assayed by flow cytometry(FCM) and polymerase chain reaction(PCR).Results One week after the cell transplantation,every main index of the peripheral blood cell counts in the gene modified group was higher than that in the control groups(P
