Evaluation on blood smear review criteria for hematology analyzer
10.3760/cma.j.issn.1673-4130.2009.11.010
- VernacularTitle:血细胞分析仪血涂片复审标准的制定及应用评价
- Author:
Jie YUAN
- Publication Type:Journal Article
- Keywords:
Blood cells;
Microscopy;
Reference standards;
Evaluation studies;
Equipment and supplies
- From:
International Journal of Laboratory Medicine
2009;30(11):1066-1067,1069
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish and evaluate blood smear review criteria for hematology analyzer,use hematology analyzer accurately,rationally and efficiently,so as to establish the review criteria suitable for our hospital.Methods The blood smear review criteria was established according to characteristics of hospitals and XE-2100 type hematology analyzer,and microscopic examination was performed on 1368 cases of blood smears.Then the concordance of test results of the hematology analyzer and manual method was analyzed,and the feasibility of the criteria was evaluated.Results The comparative results of analyzer method and blood smear method were as follows:platelet reduction (Kappa=0.95,U=35.19,P<0.0 1;with good concordance);immature granulocyte (Kappa=0.64,U:24.9,P<0.01;with acceptable concordance);atypitical lymphocyte (Kappa=0.60,U:22.2,P<0.01;with acceptable concordance);shift to the left (Kappa=0.52,U=21.98,P<0.01,with acceptable concordance);platelet aggregation (Kappa=0.60,U=17.56,P<0.01;with acceptable concordance):myeloblast (Kappa=0.39,U=13.54,P<0.01;with poor concordance): erythroblast (Kappa=0.36,U=8.24,P<0.01;with poor concordance).According to the review standard,the sensitivety.specificity and accuracy were all higher(81.9%,99.0%A and 95.5%respectively).Conclusion The review standard has high sensitivity and specificity,which indicates the specimens in accordance with the review standard should be performed microscopic examination according to the operating instruction so as to reduce missed diagnosis and misdiagnosis,improve the quality of hematological analysis.
