A comparison of the detecting methods on gene polymorphism of methylene tetrahydrofolate reductase
- VernacularTitle:亚甲基四氢叶酸还原酶基因多态性两种检测方法之比较
- Author:
Zhinong YIN
;
Ruijie LI
- Publication Type:Journal Article
- Keywords:
Methylenetetrahydrofolate reductase (NADPH);
Fluorescence;
Polymerase chain reaction
- From:
International Journal of Laboratory Medicine
2008;29(11):1003-1004,1007
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method for the detection of methylene tetrahydrofolate reductase (MTHFR) 677 C→T mutation by real-time fluorescence PCR.Methods MTHFR 677 C→T mutation was detected in 300 healthy people by the technique of polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) and real time fluorescence PCR. Sequencing was performed to confirm the accuracy of detection results. Results Three genotypes (CC, CT and TT) were found with two methods in all the 300 people. There were no significant differences in genotype frequency and allele frequency between the two groups (P>0.05).Conclusion Real-time fluorescence PCR is an accurate and rapid method for prompt grouping of MTHFR gene locus.
