Analysis of Sildenafil in blood by HPLC
- VernacularTitle:全血中西地那非的HPLC分析
- Author:
Shiyun YANG
;
Zengping YUAN
;
Jing QIAO
- Publication Type:Journal Article
- Keywords:
forensic toxicological analysis;
sildenafil;
HPLC;
SPE;
SILICA GEL
- From:
Chinese Journal of Forensic Medicine
2009;24(6):392-393,409
- CountryChina
- Language:Chinese
-
Abstract:
Objective An analytical approach has been developed for determination of sildenafil blood with high performance liquid chromatography.Methods Sildenafil was extracted by solid phase extraction cartridges from blood and was separated by resered-phase gradient chromatography with DAD detection at 230nm.The analytical column was Nova-pak C18(150×3.9mm),5.0μm and the guard column was Phenomenex C18(ODS,4.0×3.0mm,Octadecyl).The mobile phase Was gradient mixture of acetonitrile(A) and 0.25% acetonitrile aqueous solution including 0.06% trifluoroacetic acid and 0.06% triethylamine (B).Results Linearity Was checked over the concentration range 1.5～80.0μg/mL.Limits of detection was 5.0ng.Average recovery was 82.45%.Conclusion This method issimple.exact and rapid.
