Metallo-?-lactamase Produced by Carbapenem-resistant Pseudomonas aeruginosa
- VernacularTitle:耐碳青酶烯类抗菌药物铜绿假单胞菌金属酶研究
- Author:
Suan YU
;
Xiangjing ZHOU
;
Lun ZHU
;
Xiaolu JIANG
- Publication Type:Journal Article
- Keywords:
Pseudomonas aeruginosa;
Metalloenzyme;
PCR;
Sequence analysis
- From:
Chinese Journal of Nosocomiology
1994;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To study the metallo-?-lactamases of 5 carbapenem resistant Pseudomonas aeruginosa isolates,which were recovered at 2006 in the Third People's Hospital of Yueqing. METHODS K-B method was used to determine the antimicrobial agents susceptibility in 5 isolates. The minimal inhibitive concentrations (MICs) of antimicrobial agents to these strains were determined by agar dilution method. Double disk synergy test was used to detect the metallo-?-lactamase. Molecular screening for blaIMP,blaVIM,and blaSPM was carried out using PCR method. The PCR product was sequenced. RESULTS One out of the 5 carbapenem resistant P. aeruginosa was positive for MBL double disk synergy test,and confirmed to contain blaVIM-2 gene. CONCLUSIONS A blaVIM-2-producing isolate of P. aeruginosa is identified. This carbapenem-resistant isolate is all multi-drug resistant.
