Drug Resistance Analysis of Pseudomonas aeruginosa in Intensive Care Unit and Molecular Mechanism of Its Resistance to Fluoroquinolones
- VernacularTitle:重症监护病房铜绿假单胞菌的耐药性分析及其耐氟喹诺酮的分子机制研究
- Author:
Lixian WU
;
Guofu WANG
;
Chao ZHUO
;
Xingping ZHENG
- Publication Type:Journal Article
- Keywords:
Intensive care unit;
Pseudomonas aeruginosa;
Fluoroquinolone;
Mechanism of resistance
- From:
Chinese Journal of Nosocomiology
1994;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To study the Pseudomonas aeruginosas drug resistance in intensive care unit and its fluoroquinolone-resistant molecular mechanism,and provide scientific basis for rational employment of antibiotics in clinic. METHODS E test was used to determine the minimal inhibitory concentrations(MIC) of 13 antibiotics against 83 P. aeruginosa strains. Twenty-eight fluoroquinolone-resistant strains were selected with standard sensitive strain-ATCC27853 as control. The quinolone resistance-determining region(QRDR)of the gyrA and parC genes was amplified by PCR and sequenced. RESULTS The positive rate of P. aeruginosa in sputum specimen was the highest from 83 strains (71.08%). gyrA Genes of all resistant strains had an ACC to ATC mutation in codon 83,leading to the amino acid substitution of threonine for a an isoleucine and 11 high level resistant strains also showed a GAC to GGC mutation in codon 87,leading to the substitution of an aspartic acid a glycine for. In addition,14 resistant strains also had an TCG to TTG mutation in codon 87 of parC gene,leading to the amino acid substitution of a serine for a leucine. We didn't find parC gene mutation existing independent in fluoroquinolone-resistant P. aeruginosa. CONCLUSIONS Meropenem remains highly active against P. aeruginosa. But the abuse of imipenem and other fluoroquinolones leads to rise in their drug-resistance rate. Fluoroquinolone-resistance has increased rapidly,the mechanism of resistance is gene mutant. It displayed that gyrA and parC gene mutation is associated with fluoroquinolone resistance.
