Detection of qacE△1-sul1 Gene in Pseudomonas aeruginosa Isolated from Burned Patients and Its Clinical Significance
- VernacularTitle:铜绿假单胞菌烧伤患者分离株qacE△1-sul1基因检测及临床意义
- Author:
Xihao HU
;
Xiaomin XU
;
Zuhuang MI
;
Weiyun FENG
;
Youfen FAN
- Publication Type:Journal Article
- Keywords:
Pseudomonas aeruginosa;
qacE△1-sul1 gene;
PCR;
Resistance
- From:
Chinese Journal of Nosocomiology
1994;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To investigate the disinfectant-resistant gene qacE△1-sul1 in Pseudomonas aeruginosa isolated from burned patients. METHODS GNS-448 and K-B tests were performed to detect the susceptibility of 19 kinds of antimicrobial agents against these strains. Genotype was analyzed by polymerase chain reaction(PCR) and verified by DNA sequencing. RESULTS The 32 strains isolated were all resistant to ampicillin,cefuroxime,cefoxitin,SMZ/TPM. The sensitive rates to amikacin and gentamicin were 68.0%,and 46.9%,respectively,the resistant rates to imipenem and meropenem were 68.8% and 59.4%,respectively. The positive rate of gene qacE△1-sul1 was 50.0%. CONCLUSIONS The resistance of P. aeruginosa isolated from burned patients is a serious issue.There is high positive percentage of qacE△1-sul1 gene in P. aeruginosa isolated from burned patients.
