Interleukin-27 inhibits tumor angiogenesis through up-regulating expression of MIG and IP-10
- VernacularTitle:IL-27通过上调MIG和IP-10的表达抑制肿瘤血管形成
- Author:
Lihua LIU
;
Baoen SHAN
;
Shijie WANG
;
Lianmei ZHAO
- Publication Type:Journal Article
- Keywords:
IL-27;
esophageal neoplasms;
antiangiogenesis;
IP-10;
MIG
- From:
Chinese Journal of Cancer Biotherapy
1994;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the inhibitory effect of IL-27 against human tumor angiogenesis and the related mechanisms.Methods: Human esophageal carcinoma cells(Eca109/IL-27) stably transfected with IL-27 gene were injected into nude mice to establish tumor-bearing mouse model.The survival time and tumor growth were observed.IFN-? level secreted by splenocytes was measured by ELISA.Expression of VEGF and CD34 was detected by immunohistochemistry method and MVD was calculated according to CD34 level.RT-PCR was used to detect the expression of IP-10 and MIG mRNA in the tumor tissues.Results: The survival time of mice injected with Eca109/IL-27 cells was significantly longer than those of mice injected with wide type Eca109 or Eca109/LXSN(blank vector) cells(P
