Inhibitory effect of antisense human telomerase RNA on implanted hepatocellular carcinoma in nude mice
- VernacularTitle:反义端粒酶RNA基因对裸鼠肝癌移植瘤的抑制作用
- Author:
Qingshan PEI
;
Jiyong LIU
;
Xinxin SUN
- Publication Type:Journal Article
- Keywords:
antisense telomerase RNA;
implanted hepatocellular neoplasms;
proliferation;
apoptosis
- From:
Chinese Journal of Cancer Biotherapy
1994;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the inhibitory effect of antisense human telomerase RNA(hTR) gene on implanted hepatocellular carcinoma in nude mice.Methods: HepG2 cells were subcutaneously inoculated into BALB/c nude mice at the axilla to establish implanted hepatocellular carcinoma model.The retrovirus plasmid containing antisense telomerse RNA(PLXSN-hTR-BamHⅠ) was injected into the tumor(0.2 ml every time,5 times).Retrovirus plasmid containing sense telomerase RNA(PLXSN-hTR-EcoRⅠ) and normal saline were inoculated as control groups.Tumor volume was determined and the inhibitory rate was calculated.Tumor necrosis was observed by histological analysis and cell apoptosis was analyzed by terminal transferase dUTP nick end labeling(TUNEL).Results: Tumor growth in antisense hTR group was significantly inhibited compared with the two control groups.The tumor inhibitory rate(26.78%) of antisense hTR group was significantly higher than that of sense hTR group(1.93%,P
