Effect of AdIL-12 infected mesenchymal stem cells on proliferation of C6 glioma cells
- VernacularTitle:骨髓间充质干细胞表达外源性IL-12对胶质瘤C6细胞增殖的影响
- Author:
Xu ZHAI
;
Hong ZHANG
;
Xiaochuan HUO
;
Xingbo LIU
- Publication Type:Journal Article
- Keywords:
glioma,mesenchymal stem cells(MSCs),IL-12;
proliferation
- From:
Chinese Journal of Cancer Biotherapy
1994;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the influence of mesenchymal stem cells(MSC) infected by AdIL-12 on the proliferation of C6 glioma cells.Methods: The MSCs were cultured from rat bone marrow and verified by immunohistochemistry and flow cytometry.Recombinant adenovirus vectors harboring IL-12(AdEasy-IL-12) were infected into MSCs to construct AdIL-12-MSC containing exogenous IL-12.RT-PCR and Western Blotting were used to detect IL-12 mRNA and protein expression in AdIL-12-MSC.MTT method was used to detect the effect of AdIL-12-MSC supernatant on the proliferation of C6 glioma cells.Results: Exogenous IL-12 gene was effectively transfected into MSCs by recombinant adenovirus vectors.IL-12 was expressed in MSCs at both mRNA and protein levels as detected by RT-PCR and Western Blotting.The supernatant of AdIL-12-MSC significantly inhibited the proliferation of C6 glioma cells compared with MSC supernatant(P
