Aminoglycosides Modification Enzymes Genes in Pseudomonas aeruginosa Isolated from NICU Patients
- VernacularTitle:神经内科重症监护病房铜绿假单胞菌氨基糖苷类修饰酶基因研究
- Author:
Shuping ZHAO
;
Lin LI
;
Meijie JIANG
- Publication Type:Journal Article
- Keywords:
Pseudomonas aeruginosa;
Aminoglycosides modification enzymes
- From:
Chinese Journal of Nosocomiology
2006;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To investigate genes associated with aminoglycosides modification enzymes(AMEs)in Pseudomonas aeruginosa(PAE)isolated from NICU patients.METHODS Drug-resistant genes encoding AMEs such as aac(3)-Ⅰ,aac(3)-Ⅱ,aac(3)-Ⅲ、aac(3)-Ⅳ,aac(6')-Ⅰ,aac(6')-Ⅱ,aph(3')-Ⅵ,ant(3″)-Ⅰand ant(2″)-Ⅰwere detected bypoly merase chain reaction(PCR)amplification in 36 PAE isolates.The bacteria were identified by ATB.RESULTS The positive rates of aac(6')-Ⅱ,aac(3)-Ⅱ,aac(6')-Ⅰ and aph(3')-Ⅵ genes were 52.8%,47.2%,11.1% and 2.8% of 36 isolates,respectively.Drug-resistant genes encoding AMEs were detected positively in 77.8% of 36 isolates.CONCLUSIONS AMEs genes are present in high percentage of PAE isolated from NICU patients.
