Resveratrol Attenuated Reactive Oxygen Species in Injured Endothelial Cells
- VernacularTitle:白藜芦醇减轻内皮细胞损伤与活性氧的表达
- Author:
Yanghui SHEN
;
Pengli ZHU
;
Dean JIA
;
Jingming RUAN
;
Huizhen YU
;
Hui CHEN
- Publication Type:Journal Article
- Keywords:
Resveratrol;
Atherosclerosis;
Endotheliocyte;
Reactive oxygen species
- From:
Chinese Journal of Hypertension
2007;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Background Resveratrol has been unanimously recognized as an cardiovascular protective substance in red wine. It has been speculated that the anti-atherosclerosis effect of resveratrol is ascribed to its powerful anti-inflammatory effect. Objective To investigate the effects of resveratrol on injured human umbilical veno-endothelial cells (HUVEC) and the reactive oxygen species(ROS) production induced by TNF-? or soluble CD40L (sCD40L). Methods Cultured HUVEC were pre-incubated with resveratrol(1-50 ?mol/L) for 2 hours and then treated with TNF-?(10 ?g/L) or sCD40L?(10 ?g/L) for another 4 hours. MTT assay was used to detect proliterative activity of HUVEC. Immunofluorescence microscopy was used for determination of ROS expression. Results Both TNF-? and sCD40L impaired HUVEC proliferation (-32.7% and -26% vs control,P
