Influences of recombinant decorin gene transfection on p21~(WAF1/CIP1) of HepG2 hepatoma cells
- VernacularTitle:重组核心蛋白聚糖转染对HepG2细胞p21~(WAF1/CIP1)表达的影响
- Author:
Yali WANG
;
Yucheng ZHANG
;
Zhenwu DU
;
Guizhen ZHANG
- Publication Type:Journal Article
- Keywords:
decorin;
p21WAF1/CIP1;
HepG2;
transfection
- From:
Journal of Jilin University(Medicine Edition)
2006;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To transfect a recombinant pcDNA3.1-DCN into HepG2 cells and detect the expressions of decorin(DCN) and p21WAF1/CIP1 so as to investigate the mechanism of tumor suppression of DCN.Methods HepG2 cells were divided into pcDNA3.1-dec-HepG2 group(transfected group) and pcDNA3.1-HepG2 group(control group).After the recombinant pcDNA3.1-DCN and pcDNA3.1 were transfected into HepG2 hepatoma cells by liposome,the stably transfected cell lines were established using G418 screening.RT-PCR method was used to detect the expressions of DCN and p21WAF1/ CIP1 mRNA;Western blotting method was used to detect the expressions of DCN and p21WAF1/CIP1 protein;immunohistochemistry was performed to detect the expression of DCN protein.Results Compared with control group,the expressions of DCN and p21WAF1/CIP1 mRNA were significantly increased in transfected group by RT-PCR(P
