Effect of ionizing irradiation on expressions of TLR4 and adaptor protein MyD88 in peritoneal macrophages of mice
- VernacularTitle:电离辐射对小鼠腹腔巨噬细胞TLR4和转接蛋白MyD88表达的影响
- Author:
Hui GAO
;
Haiyu ZHANG
;
Yuxing SHAN
- Publication Type:Journal Article
- Keywords:
radiation,ionizing;
macrophages;
Toll-like receptor 4;
myeloid differetiation factor 88
- From:
Journal of Jilin University(Medicine Edition)
2006;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of ionizing radiation on the expressions of TLR4 and adaptor protein MyD88 in mouse peritoneal macrophages in the Toll-mediated signal transduction pathways.Methods 160 male Kunming white mice were randomly divided into 16 groups(ten each group):sham-irradiation and five groups after 4,8,16,24 and 48 h X-ray irradiation for time-course experiments;sham-irradiation and nine groups after 0.05,0.075,0.100,0.200,0.500,1.000,2.000,4.000,6.000 Gy irradiation for dose-effect experiments.Flow cytometry was used to detect the expressions of TLR4 and MyD88 after whole body irradiation (WBI) with X-rays.Results Both of TLR4 and Myd88 expressed more than shamirradiation after 0.075 and 2.000 Gy WBI for 4 h,and the expressions of them reached the peak at 16 h or 4 h after WBI(P
