Effect of down-regulating survivin expression by RNAi on growth suppression and promotion on apoptosis of human hepatocellular carcinoma cells
- VernacularTitle:RNA干涉下调survivin基因对人肝癌细胞的生长抑制及促凋亡作用
- Author:
Jing LI
;
Zheng JIANG
;
Li CHEN
;
Haibo SUN
- Publication Type:Journal Article
- Keywords:
hepatocellular carcinoma;
RNA inter;
apoptosis;
survivin
- From:
Journal of Jilin University(Medicine Edition)
2006;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of down-regulating survivin expression by RNAi on growth suppression and promotion on apoptosis of human hepatocellular carcinoma cells,in order to supply an effective target and technique for liver cancer gene therapy.Methods The recombinant plasmid of pSU6-si-survivin was constructed,which was transfected into Bel7402 cells.Mock group,negative group and treated group were set up.Using MTT assay the proliferation of transfected cells was investigated,the survivin protein expression level was determined by Western blotting and immunohistochemical staining.Transcription of survivin was detected by semi-quantitative RT-PCR.Flow cytometry(FCM) and AO/EB double dyeing were used to examine apoptosis in transfected cells.Results MTT assay demonstrated that the cell growth inhibition rates(%) of transfected cells in treated group at 24,48 and 72 h were 21.43?1.67,39.07?3.80,and 59.72?7.05,there were significant differences compared with negative group(P
