Effect of platelet activation on the levels of VEGR,TGF-?_1 and PDGF in apheresis platelets
- VernacularTitle:血小板活化对单采血小板制剂中VEGF、TGF-?_1和PDGF含量的影响
- Author:
Jing LI
;
Ruqing LI
;
Xin WANG
- Publication Type:Journal Article
- Keywords:
Plasma;
Apheresis platelets;
Vascular endothelial growth factor;
Transforming growth factor ?1;
Platelet derived growth factor CD62p;
ELISA;
Flow cytometry
- From:
Chinese Journal of Blood Transfusion
1988;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the changes of vascular endothelial growth factor (VEGF),transforming growth factor-?1 (TGF-?1) and platelet derived growth factor (PDGF) in plasma and apheresis platelets with or without activation by shaking. Methods A total of 10 units of double therapeutic dose platelets were collected and each unit was divided into 3 groups to take samples of plasma (3ml/person,as control group),fresh apheresis platelets (5ml/person,as experimental group1) and activated apheresis platelets (platelets stored by shaking at 22 ℃ for 5 days,5ml/person,as experimental group2). The levels of VEGF,TGF-?1 and PDGF in the samples of the 3 groups were measured by ELISA. The expression of CD62p in 2 apheresis experimental groups was measured by flow cytometry. The differences between the two groups were compared. Results The levels of VEGF,TGF-?1 and PDGF,which were (410.95?95.07) pg/ml,(91.15?19.50) ng/ml and (12.60?2.06) ng/ml,respectively,in group 1 were markedly higher than those in the plasma control group,which were 149.09?28.11,37.38?10.73 and 3.28?0.79,respectively (P
