Cloning of the novel putative apoptosis-related gene of Spirometra erinacei (Order Pseudophyllidae).
10.3347/kjp.2006.44.3.233
- Author:
Soo Ung LEE
1
;
Sun HUH
Author Information
1. Department of Parasitology, College of Medicine, Hallym University, Chuncheon 200-702, Korea. shun@hallym.ac.kr
- Publication Type:Brief Communication ; Research Support, Non-U.S. Gov't
- Keywords:
Spirometra erinacei;
cestoda;
apolysis;
apoptosis;
cDNA library
- MeSH:
Spirometra/*genetics/growth & development/physiology;
Molecular Sequence Data;
Gene Library;
Cloning, Molecular;
Caspases/genetics;
Caenorhabditis elegans Proteins/genetics;
Base Sequence;
Apoptosis/*genetics;
Animals;
Amino Acid Sequence
- From:The Korean Journal of Parasitology
2006;44(3):233-237
- CountryRepublic of Korea
- Language:English
-
Abstract:
We postulated that apolysis was processed in accordance with apoptotic changes occurring in a cestode, Spirometra erinacei (Pseudophyllidea). We cloned the novel putative apoptosis-associated gene from S. erinacei via screening of a S. erinacei cDNA library with a ced-3 gene (activator of apoptosis) probe from Caenorhabditis elegans. We identified a 261-bp cDNA sequence, which encodes for an 86-amino acid protein. The cloned gene expression was observed in the neck and gravid proglottids via Northern blotting, using cloned cDNA inserts as probes, but the clone was not expressed in any of other tissues. We suggest that this gene may be involved in the apolysis of S. erinacei during normal tissue development and differentiation in cestode parasites.
