Real-time Fluorescent Quantitative PCR Assay for Early Diagnosis of Invasive Aspergillosis in Patients after Allo-hematopoietic Stem Cell Transplantation
- VernacularTitle:荧光实时定量PCR方法在异基因造血干细胞移植后侵袭性曲霉菌感染早期诊断中的价值
- Author:
Yuqian SUN
;
Lanping XU
;
Xiaojun HUANG
- Publication Type:Journal Article
- Keywords:
Invasive aspergillosis;
Real-time fluorescent quantitative PCR;
Allo-hematopoietic stem cell transplantation
- From:
Chinese Journal of Nosocomiology
2006;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To evaluate the role of real-time fluorescent quantitative PCR assay in the early diagnosis of invasive aspergillosisi(IA) after allo-hematopoietic stem cell transplantation.METHODS All 123 serum samples from 18 IA patients and 38 no-invasive fungal infection(IFI) patients were detected by TaqMan-based real-time PCR to explore the cutoff of this array,while the primer and probe could amplificate five major Aspergillus species including A.fumigatus,A.flavus,A.niger,A.terreus and A.oryzae.RESULTS The optimal value could be acheived while the cutoff was set as two or more consecutive sample DNA≥15pg/?l in a week,and the sensitivity and specificity was 94.4% and 100.0%,respectively.CONCLUSIONS Real-time fluorescent quantitative PCR is helpful in early diagnosis of invasive aspergillosis.
