Techniques for orthotopic liver transplantation in rats
- VernacularTitle:大鼠原位肝移植手术技巧探讨
- Author:
Ming CAI
;
Tao PAN
;
Hongzhou LI
;
Zhishui CHEN
- Publication Type:Journal Article
- Keywords:
Orthotopic liver transplantation;
Double-cuff technique;
Animal model;
Rat
- From:
Journal of Medical Postgraduates
2004;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the surgical techniques for establishing the rat model of orthotopic liver transplantation.Methods: On the basis of the double-cuff technique of Kamada,we improved the techniques for the separation and perfusion of the donor liver,the shearing and anastomosis of the superior and inferior caval veins,and the anastomosis of the bile duct.Results: Of the 40 rats that underwent orthotopic liver transplantation,80% survived longer than 24 hours and 70% over 7 days.Conclusion: With extreme patience and carefulness,the operator can successfully establish the rat model of orthotopic liver transplantation by shortening the anhepatic phase with skillful surgical techniques.
