The Monolayer Culturing of The Neural Stem Cell Clone and Its Qualification
- VernacularTitle:大鼠胚胎神经干细胞单克隆化及单层化培养和鉴定
- Author:
Daofang DING
;
Sanli XING
;
Mingming ZHOU
;
Houyan SONG
- Publication Type:Journal Article
- Keywords:
neural stem cells, cloning, Nestin, Sox2, GFAP, Tuji
- From:
Progress in Biochemistry and Biophysics
2006;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
The primary neural stem cells were isolated from SD rat and formed the neuropheres, the neuropheres were passaged and planted on the dish coated with 0.1% gelatin, the colony was picked up under the microscope, then dispersed and cultured, to obtain the clone proliferated from one cell, passaging and picking up the cells 5～6 times at least. The NSC and its differentiated cells were identified with the marker genes respectively. The results showed that the neural stem cells were isolated from the SD rat embryos and the real clone were obtained by picking up the cells again and again, and then cultured in the form of monolayer. The marker genes of the neural stem cells and its differentiated cells could be detected at last. It will provide the rat model the resource of the cells for the treatment and the basic research for the morphology standard.
