Sodium ferulate inhibits neurons injury mediated by A?_(25-35)-induced macrophage activation of mice
- VernacularTitle:阿魏酸钠抑制A?_(25-35)激活小鼠巨噬细胞引起的神经细胞损伤
- Author:
Suyan YAO
;
Yin JIN
;
Deyu ZHENG
- Publication Type:Journal Article
- Keywords:
sodium ferulate;
A?25-35;
TNF-?;
P38 MAPK
- From:
Basic & Clinical Medicine
2006;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To examine the effect and mechanism of sodium ferulate inhibiting P38 MAPK in macrophage on neurotoxicity which was mediated by fribrilar-amyloid-induced.Methods The isolated peritoneal macropohages were cultured.P38 MAPK protein in nuclear extracts was analyzed by Western blot and production of tumor necrosis factor-?(TNF-?) was detected by ELISA.For neuron-macrophage co-cultures,Microtubute-associated protein-2(MAP-2) expression was detected by immunocytochemical technique.The level of LDH in the medium was measured.Results The production of TNF-? and P38 MAPK protein in nuclear extracts increased significantly by incubation with A?25-35.LDH efflux in neuron-macrophage co-culture medium increased and MAP-2 expression decreased significantly(P
