Establishment and characterization of a healthy donor's ??T cell clone
- VernacularTitle:一株健康人??T细胞克隆的建立及鉴定
- Author:
Xiaojuan HE
;
Ning KANG
;
Hui CHEN
;
Lianxian CUI
;
Wei HE
- Publication Type:Journal Article
- Keywords:
??T cells;
clone;
limit dilution
- From:
Basic & Clinical Medicine
2006;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective Establishment and characterization of healthy donor's ??T cell clones.Methods ??T cells were cloned by limiting dilution after positive sorting with 60Co irradiated allogeneic PBMC as feeder cells.Flow cytometry analysis and molecular biology technique were then used to identify ??T cell clones.MTT assay was used to verify their proliferation after incubated with epitope peptides recognized by ??T cells.Results A ??T cell clone had been established.The subtype of this clone was V ?9 V ?2 without expression of CD4 and CD8.Further studies indicated that epitope peptide EP6 could not only specifically bind to ??T cell clone but also trigger the proliferation of ??T cell clone.Conclusion A healthy donor's ??T cell clone was successfully established,which laid a solid foundation for further study on ??T cells.
