Reference genes selection in mRNA analysis of aging rat tissues
- VernacularTitle:老年大鼠组织mRNA分析中内参基因的选择
- Author:
Wenjing YE
;
Xiaomin ZHAO
;
Peiqiang HOU
;
Hongxin CAI
;
Zuoli XIA
- Publication Type:Journal Article
- Keywords:
HKG;
reference genes;
real-time reverse transcription PCR;
mRNA normalization
- From:
Basic & Clinical Medicine
2006;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the proper inner control genes suitable for mRNA expression level comparison of aging rat tissues.Methods Real-time reverse transcription PCR was used to examine in aging rat tissues the expression level of G3pd(glyceraldehyde-3-phosphate dehydrogenase),ACTB(?-actin),H3f3b(H3 histone,family 3B),Arbp(acidic ribosomal phosphoprotein P0)and 18S(18S ribosomal RNA).Results The most stably expressed housekeeping gene in aging rat kidney was ACTB,in heart and lung G3pd showed the minimum variation;Arbp expression was the most stable one in different tissues.Conclusion For aging rat intra-tissue mRNA normalization at least two housekeeping genes should be used: one is the ribosomal RNA gene 18S and another one is Arbp.
