Thalidomide inhibits TGF-?1-induced transdifferentiation of human fetal lung fibroblast cell line to myofibroblast
- VernacularTitle:沙利度胺抑制TGF-?1诱导的人胚肺成纤维细胞系转分化为肌成纤维细胞
- Author:
Zhi WANG
;
Lidan ZHAO
;
Xuan ZHANG
;
Fulin TANG
;
Mengxue YU
- Publication Type:Journal Article
- Keywords:
human fetal lung fibroblast;
myofibroblast;
TGF-?1;
thalidomide
- From:
Basic & Clinical Medicine
2006;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of Thalidomide(THD)on transdifferentiation of human fetal lung fibroblast(HFL-F) to myofibroblast(MF) induced by Transforming Growth Factor-?1(TGF-?1) and the effects on trans differentiated MF.Methods HFL-F to MF trans-differentiation was induced with 5 ?g/L TGF-?1.The effect of 50 ?g/L THD on HFL-F to MF transdifferentiation was evaluated by measuring hydroxyproline(HYP) content with alkaline hydrolysis colorimetry,?-smooth muscle actin(?-SMA) protein with Western Blot,?-SMA and collagen Ⅲ(COL Ⅲ) mRNA with semiquantitative RT-PCR.Results THD inhibited TGF-?1 induced up-regulation of HYP and COLⅢ mRNA expressions(all P0.05).For HFL-F treated with 5 ?g/L TGF-?1 for 96 h,THD inhibited COLⅢ mRNA expression(P
