Inhibitory effect of metformin on TNF-? expression in hepatic tissue of OLETF rats
- VernacularTitle:二甲双胍抑制OLETF大鼠肝组织TNF-?表达
- Author:
Aimei DONG
;
Xiaohui GUO
;
Wei WANG
- Publication Type:Journal Article
- Keywords:
metformin;
TNF-?;
insulin resistance;
fatty liver disease
- From:
Basic & Clinical Medicine
2006;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the TNF-? expression in hepatic tissue of Otsuka Long-Evans Tokushima Fatty(OLETF) rats(an insulin resistance model) with abnormalities of glucose-lipid metabolism and effect of metformin on TNF-? expression.Methods OLETF rats were randomly assigned to two groups(OLETF and OLETF/M).The OLETF/M group was treated with metformin [100 mg/(kg?d)],the OLETF group and age-matched LETO(nondiabetic Long-Evans Tokushima Otsuka rats) group were fed with placebo.Twenty-two weeks after treatment,we analyzed hepatic triglyceride level and measured expression of TNF-? by western-blot(protein) and quantitative RT-PCR(mRNA).Results Twenty-two weeks after treatment,the level of fasting glucose,insulin,triglyceride,free fatty acids(FFA) and hepatic triglyceride increased significantly in OLETF group as compared with LETO group.Western-blot method showed upregulated expression of TNF-? protein in OLETF group(1.57-fold vs LETO group,P
