Expression of protease activated receptors in mast cells
- VernacularTitle:蛋白酶激活受体在鼠肥大细胞的表达
- Author:
Huiyun ZHANG
;
Shaoheng HE
- Publication Type:Journal Article
- Keywords:
protease activated receptors(PARs);
mast cell;
reverse transcription polymerase chain reaction(RT-PCR);
flowcytometry;
immunofluorescent staining for cells
- From:
Chinese Journal of Clinical Laboratory Science
2006;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of protease activated receptors(PARs)in mast cells.Methods Reverse transcription polymerase chain reaction(RT-PCR),flowcytometry and immunofluorescent cell staining were used to detect the expression of PARs in mast cell lines P815 and MC/9 at the levels of protein and mRNA.Results Both the P815 and MC/9 of mast cell lines expressed PAR-1,PAR-2,PAR-3 and PAR-4 at either protein or mRNA level.Conclusion The expression of all the four PARs in mast cells were detectable,which may be of significance for the further study on the function of PARs in mast cells.
