Antibiotic-resistant Genotypes in AmpC and ESBLs-producing Klebsiella pneumoniae
- VernacularTitle:产AmpC酶和ESBLs肺炎克雷伯菌的耐药性分析与耐药基因检测
- Author:
Dongguo WANG
;
Tieli ZHOU
- Publication Type:Journal Article
- Keywords:
Klebsiella pneumoniae;
Cephalosporinase C;
Extended-spectrum beta-lactamases;
Genotypes;
AMEs
- From:
Chinese Journal of Nosocomiology
1994;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To study antibiotic resistance and drug-resistant genes in AmpC and ESBLs-producing Klebsiella pneumoniae(KPN) in Wenzhou and Taizhou of Zhejiang Province.METHODS Antimicrobial susceptibilities of AmpC and ESBLs-producing KPN were tested by broth dilution motheds and VITEK 60 and VITEK 32;the genotypes of extended-spectrum beta-lactamases(ESBLs),cephalosporinase C(AmpC) and Genotypes AMEs were analyzed by polymerase chain reaction(PCR).RESULTS All tested KPN strains were susceptible to imipenem and resisitant to more other drugs.TEM genes were amplified in 20(100%) strains,CTX-MⅠgenes were examined in 18(90%) strains,SHV genes were examined in 2(10%) strains,DHA genes were tested in 17(85%) strains,MIR genes were examined in 3(15%) strains and 3 kinds of AMEs genes were tested in 18(90%) strains.CONCLUSIONS AmpC and ESBLs-producing KPN is multiple drug-resisitant,in which 2 to 6 resistant genes exist,Imipenem is the best drug.
