Establishment of a rabbit model of femoral head avascular necrosis using a large dose of hormone
- VernacularTitle:大剂量激素诱导建立兔股骨头缺血性坏死模型
- Author:
Debao LIU
;
Haifu BU
;
Binjie GUI
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2007;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND: There is not any experimental model that can reflect the pathological change of hormone-induced avascular necrosis of the femoral head in a good way nowadays, which is unfavourable to the further observation of its pathogenesis. OBJECTIVE: The model of the avascular necrosis of the femoral head is induced by giving a large dose of dexamethasone into rabbits, which provides the animal experimental models for the study of clinical pathogenesis. DESIGN, TIME AND SETTING: The randomized controlled animal experiment was performed at the Animal Laboratory, Affiliated Hospital of Anhui Medical University from June 2004 to August 2005. MATERIALS: Sixteen healthy adult New Zealand albino rabbits (50% female and 50% male), weighing 3.0-3.2 kg were selected for this study. Dexamethasone was provided by Jiangsu Lianshui Medical Co., Ltd. etalon: 5 g/L. METHODS: Rabbits were randomly divided into two groups: model and control groups. Dexamethasone of 2.5 mg/kg per day was injected into each rabbit of the model group, and saline of 2 mL was injected into each rabbit of the control group for 6 weeks. MAIN OUTCOME MEASURES: Serum lipid and prothrombin time (pt) were tested before and after the experiment, and the histopathological change was observed with radiography, optical microscrope and transmission electron microscopy at 2, 4 and 6 weeks of dexamethasone administration. RESULTS: Compared with the control group, in the model group cholesterin levels were significantly increased at 2 weeks (P
