Anti-tumor and hypoxia regulatory effect of HIF-1 a specific siRNA in transplanted VX2 tumor
- VernacularTitle:HIF-1?特异性siRNA对VX2移植瘤组织乏氧状态的调节及其抗瘤效应
- Author:
Jianxin DOU
;
Ruimei REN
- Publication Type:Journal Article
- Keywords:
hypoxia inducible factor;
RNA interference;
VX2 tumor;
gene therapy;
positron emission computed tomography
- From:
Chinese Journal of Cancer Biotherapy
2006;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the anti-tumor and hypoxia regulatory effect of HIF-1?siRNA in tumor tissues. Methods:The HIF-1?siRNA adenovirus vectors targeting HIF-1?were constructed and the rabbit VX2 tumor model was established.The constructed vectors were injected into rabbits through ear vein;noninterference sequence was injected as control.PET(positron emission computed tomography)was used to observe the changes of hypoxia and necrosis in tumor tissues.The tumors were removed and the tumor volumes were measured.H-E staining was used to observe the degree of the necrosis;RT-PCR was used to detect the change of HIF-1?expression in tumor tissues.Results:The recombinant HIF-1?siRNA adenovirus vectors were successfully constructed,with a titre of 1.1?10~(10)pfu/mL.PET imaging showed that the SUV of ~(18)F-FDG was significantly decreased in HIF-1?siRNA injected group than that in the control group ([3.51?0.36]%vs[8.73?0.83]%,P
