Influence of Bifidobacterium on expression of HIF-1?and VEGF in rabbit VX2 tumor
- VernacularTitle:双歧杆菌对兔VX2瘤HIF-1?和VEGF表达的影响
- Author:
Xiaoshan CAI
;
Ruimei REN
;
Shinuan ZHANG
- Publication Type:Journal Article
- Keywords:
Bifidobacterium adolescence;
rabbit VX2 tumor;
hypoxia-inducible factor-1?(HIF-1?);
VEGF;
immunohistochemistry
- From:
Chinese Journal of Cancer Biotherapy
2006;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the influence of Bifidobacterium adolescence on the expression of HIF-1?and VEGF in rabbit VX2 tumor.Methods:Rabbit VX2 tumor models were established and were divided into two groups(n= 10).Animals in the experimental group were injected with Bifidobacterium adolescence and those in the control group received normal saline via ear vein.Animals were sacrificed and the liver,kidney,spleen,heart,lung and tumor tissues were removed,cultured and Gram stained.The changes of tumor tissue were observed by H-E staining;immunohistochemistry was used to detect the expression of HIF-1?and VEGF in tumor tissues.Results:Bifidobacterium adolescence could target the tumor tissue.H-E staining showed the necrotic area was obviously enlarged after treatment with Bifidobacterium. Immunohistochemistry demonstrated that the positive rates of HIF-1?and VEGF in the experimental group were significantly lower than those of the control group(P
