Morphological alterations of denervated muscle following muscular transplantation with the pedicle of blood vessels and nerves
- VernacularTitle:带神经血管肌束分点植入后失神经肌肉形态学改变
- Author:
Aiguo WANG
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2008;0(53):-
- CountryChina
- Language:Chinese
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Abstract:
BACKGROUND: Denervated muscular atrophy is refractory in clinical treatment. To date, there is still no effective treatment for this disease. OBJECTIVE: To observe the influence of two-division muscular transplantation with the pedicle of blood vessels and nerves on denervated muscular atrophy so as to explore a new thought for denervated muscular atrophy. DESIGN, TIME AND SETTING: Controlled animal trial was performed at the Department of Hand Surgery, Drum Tower Hospital of Nanjing University of Medical College between July 2006 and December 2007. MATERIALS: Thirty clean-grade healthy Wistar rats, weighing 250 g, irrespective of gender, were selected. METHODS: Gastrocnemius muscle denervation models were established in both lower extremities. At the experimental side, two incisions were made in 1/3 gastrocnemius muscle of the right side, and the pedicle of the blood vessels and nerves was transplanted into the lateral head of gastrocnemius. While in the control side (left side), gastrocnemius muscle was only disconnected. Samples were harvested at 4, 8, and 12 weeks postoperatively. MAIN OUTCOME MEASURES: Muscular appearance and ultrastructure were observed; muscle fiber circumference and cross-section area and collagen fiber content were measured. RESULTS: The gastrocnemius muscle at the experimental side appeared plump and elastic with acceptable color, while the muscle became atrophy with pale appearance at the control side. Circumference and cross-section area of the muscle fibers at the experimental side were significantly larger than the control side (P
