Effect of hair follicle unit transplantation on wound healing
- VernacularTitle:毛囊单位移植促进创面愈合
- Author:
Xiao SHEN
;
Fazhi QI
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2008;0(53):-
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND: After trauma, dermal sheath cells can directly participate in the repair of dermal injury or transfer into fibroblasts, which join in skin repair. OBJECTIVE: To establish models of rat hair follicles, to investigate and analyze the histology and ultrastructure of dermal component during wound healing, and to explore the potential of hair follicle tissues for dermal healing. DESIGN, TIME AND SETTING: The control animal experiment was conducted at the Zhongshan Hospital Affiliated to Fudan University from September 2007 to April 2008. MATERIALS: Eighteen Sprague Dawley (SD) rats weighing 200-250 g were used to prepare models of hair follicle units and mini-epidermis transplantation on wound. METHODS: Rat hair follicle units were cut from lateral vibrissa pad skin. Incision was sutured. Vibrissa pad hair follicles were cut with a sharp tool to collect hair follicle with papilla, surrounding tissues and partial surface. Hair follicle units were implanted into the wound, 5-7 units in each wound. Epidermis and some dermic skin were obtained from full-thickness skins of rats in the mini-epidermis transplantation group, and then trimmed into 3?3 mm size, 5-7 mini-epidermis in each wound. MAIN OUTCOME MEASURES: The wound specimens were obtained at the 2nd, 3rd, 4th, 5th weeks postoperatively. Hematoxylin-eosin stain, Picric-sirius red stain and AWMSEM (alkaline water maceration scanning electron microscope) were performed, and observed under a polarization microscope and scanning electron microscope. The images were processed and analyzed with computerized image analysis system. RESULTS: No significant difference in type and quantity of collagen matrix was detected in both groups at the early phase after wounding. The type Ⅲ collagens kept reducing as time elapsed, but type Ⅰ increased. At 5 weeks after wounding, the ratios of types Ⅰ and Ⅲ collagen increased in both groups, especially in the control group. Significant differences were detected between both groups (P
