Isolation and purification of human plasma coagulation Factor Ⅺ
- VernacularTitle:人血浆凝血因子Ⅺ的纯化
- Author:
Yuxiang HOU
;
Fangzhao LIN
;
Xiaopu XIAO
- Publication Type:Journal Article
- Keywords:
Coagulation factor XI;
Isolation;
Purification
- From:
Chinese Journal of Blood Transfusion
2008;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To concentrate coagulation Factor Ⅺ(FⅪ)from human plasma.Methods FⅪwas isolated and purified from human plasma via two-step chromatography,including CM-Sepharose fast flow ion exchange and Heparin CL-6B affinity chromatography.Results The recovery rate of FⅪ was(21.02?5.04)%,the specific clotting activity of purified FⅪ was(17.59?1.96) U/mg,and the purification factor was(1162.29?129.64) fold(n=7).Conclusion The two-step chromatography is effective in concentrating FⅨ.
