Study of the genetic and polymorphism of human platelet antigens 1—6,15 in unrelated volunteer blood donors,Guangzhou
- VernacularTitle:广州地区无偿献血者HPA-1—6,15基因分型及频率调查
- Author:
Xin YE
;
Yongshui FU
;
Guangping LUO
- Publication Type:Journal Article
- Keywords:
Human platelet antigen(HPA);
Gene frequencies;
Polymorphism;
Sequence specific primers
- From:
Chinese Journal of Blood Transfusion
2008;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the polymorphism of human platelet antigens among unrelated Guangzhou blood donors with PCR-SSP,in order to provide basic data for population studies and clinical transfusion practice.Methods Blood samples from 706 unrelated blood donors in Guangzhou were genotyped for each of the HPA1—6,15 systems by PCR-SSP.Gene frequencies and genotype frequencies were analyzed by statistical methods.Results HPA-3 and-15 had the greatest heterozygosity with a gene frequency of 0.2918,0.4830,0.2252 for HPA-3a/a,HPA-3a/b,HPA-3b/b,and 0.2691,0.5170,0.2139 for HPA-15a/a,HPA-15a/b,HPA-15b/b.The a/a homozygosity was predominant in HPA-1,-2,-4,-5,with a frequency ranged from 0.9583 to 0.9993,while HPA b/b was not found among them.The frequency of HPA-lb and HPA-4b was very low,which was 0.0028 and 0.0007,respectively.In our study,HPA-1 frequency was significantly different from that of the north Chinese,English,and American Indian(P
