Rhein improves insulin sensitivity of diabetic rats by increasing the protein expression levels of PPAR-? and GluT-4 in adipose tissue
- VernacularTitle:大黄酸上调糖尿病大鼠脂肪组织PPAR-?及GluT-4表达并改善胰岛素敏感性
- Author:
Cheng CHI
;
Miaomiao JIN
;
Yiming MU
- Publication Type:Journal Article
- Keywords:
Insulin sensitivity;
Rhein;
Adipose tissue;
Peroxisome prliferator activated neceptor;
Glucose tronoporter
- From:
Chinese Journal of Diabetes
2008;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the mechanism for rhein to improve the insulin sensitivity in diabetes mellitus. Methods Male Wistar rats were randomly divided into normal control group (NC,n=15) and diabetic group (DM,n=40) induced by STZ and high-fat diet.Diabetic rats were randomly divided into diabetic control group (DM-C,n=15) and diabetic group treated with rhein (DM-T,n=15).Then DM-T group was given intragastric administration with rhein at the dose of 100 mg/ (kg-1?d-1) for 11 weeks.FBG,fasting serum insulin (FIns),triglyceride (TG),total cholesterol (TC),insulin sensitivity index (ISI) and other biochemical parameters in each group were measured.The protein expressions of PPAR-? and GluT-4 were determined by immunohistochemistry and Western blot. Results At the end of the 17th week,compared with NC group,DM-C group showed higher FBG and TG,lower ISI,lower protein expressions of PPAR-? and GluT-4 in the adipose tissue.Compared with DM-C group,DM-T group showed lower FBG [(15.73?3.08 vs 22.57?3.23) mmol/l,P
