The changes of insulin sensitivity and glucose-lipid metabolism in mice with TNF-? induced insulin resistance
- VernacularTitle:TNF-?诱导的胰岛素抵抗小鼠胰岛素敏感性及糖脂代谢的变化
- Author:
Lingyun OUYANG
;
Ling LI
;
Gangyi YANG
- Publication Type:Journal Article
- Keywords:
Tumor necrosis factor;
Insulin resistance;
Glucose-lipid metabolism;
Insulin clamp
- From:
Chinese Journal of Diabetes
2008;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of tumor necrosis factor-alpha (TNF-?) on insulin sensitivity and glucose-lipid metabolism in TNF-?-induced IR mice. Methods Male C57BL/6J mice were given an intraperitoneal injection of TNF-? (H group,6?g/kg; M group,3?g/kg; L group,1?g/kg;twice daily) and saline (NC group) for 7 days. The plasma glucose and insulin were assayed during intravenous glucose tolerance test (IVGTT) and hyperinsulinemic-euglycemic clamp combined with 3-[3H] glucose as a tracer was carried out. Results After TNF-? treatment,fasting blood glucose (FBG),plasma insulin and free fatty acids (FFA) were significantly elevated in H group compared with NC,L and M groups (P
