New Method to Prepare Acellular Matrix of the Penile Cavernous Body
- VernacularTitle:一种新的脱细胞阴茎海绵体基质的制备方法
- Author:
Yongyan CUI
;
Hui ZHU
;
Hui LI
- Publication Type:Journal Article
- Keywords:
Acellular matrix;
Penile cavernous body;
Matrix
- From:
Chinese Journal of Minimally Invasive Surgery
2005;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To find a new method to prepare the acellular matrix of the penile cavernous body. Methods Intact specimens of the penile cavernous body were collected from healty adult rabbits,treated with Triton-X100 and NH3?H2O,and then stained with HE.Light microscopy was employed to observe the acellular cells.Results Acellular matrix of the penile cavernous body was obtained after a 25-day treatment with Triton-X100 and NH3?H2O.The matrix was intact.HE staining showed that the matrix was composed of regularly arranged elastic fibers with wide intra-spaces without any cells inside.Conclusion Acellular matrix of the penile cavernous body can be prepared by using Triton-X100 and NH3?H2O.