Influence of Toll-like receptor 4 on inflammatory media in the lungs of mice infected with chlamydia pneumoniae
- VernacularTitle:TOLL样受体4对肺炎衣原体感染小鼠肺组织炎性递质的影响
- Author:
Yingjian SONG
;
Jing DONG
;
Yi SHI
- Publication Type:Journal Article
- Keywords:
Toll-like receptor 4;
Chlamydia pneumoniae;
Tumor necrosis factor-?;
Interleukin-10;
Mouse;
Lung infection
- From:
Journal of Medical Postgraduates
2003;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the role of Toll-like receptor 4(TLR4) in the inflammatory reaction in the mouse model of Chlamydia pneumoniae(Cpn) infection.Methods: We divided 60 TLR4 mutant C3H/HeJ and 60 wild type C3H/HeN male mice into 4 groups: a mutant control(M),a mutant infection(M+I),a wild type control(W) and a wild type infection(W+I) group,each randomly assigned to 5 scheduled time points(1 d,4 d,7 d,14 d and 21 d).The animals in the M and W groups were intranasally inoculated with 2-sucrose phosphate(2SP) buffer,while those in the M+I and W+I groups with Cpn.All the mice were killed and their lung tissues were sampled on the above time points.The levels of TNF-? and IL-10 in the lung tissues were detected by enzyme-linked immunosobent assay(ELISA),histopathological examinations were performed,and the wet / dry weight(W / D) ratio was determined.Results: After Cpn inoculation,the lung tissues were characterized by patchy interstitial pneumonitis,predominantly infiltrated with neutrophil leukocytes in the earlier(7 d) stage and with lymphocytes in the later(14 d) stage.In the M+I group,the levels of TNF-? were elevated at 1 d([0.78?0.06] pg/mg),peaked at 4 d([1.22 ? 0.12] pg/mg) and then began to decrease,but was still higher than normal at 21 d;they were(1.10 ? 0.12,0.72 ? 0.12 and 0.52 ? 0.10) pg/mg at 7,14 and 21 d,significantly lower than in the W+I group([1.30 ? 0.16],[1.01 ? 0.19] and [0.71 ? 0.08] pg/mg,P
