Rapid Identification of Staphylococci in Positive Blood Culture Bottles by PCR
- VernacularTitle:PCR法快速检测阳性血培养瓶中葡萄球菌属
- Author:
Yizheng ZHOU
;
Xiangyang LI
;
Xiaoyan QIU
;
Qianjun FU
;
Jinhong YANG
- Publication Type:Journal Article
- Keywords:
Staphylococcus;
Polymerase chain reaction;
Meticillin;
Drug resistance
- From:
Chinese Journal of Nosocomiology
2006;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To establish a method of polymerase chain reaction(PCR) for detecting staphylococci in positive blood culture bottles.METHODS Genomic DNA in 493 positive blood culture bottles was extracted by guanidine hydrochloride and benzenemethanol,then genes 16S rRNA,ssa and mecA were amplified by PCR to identify staphylococci.Finally,the results of PCR were compared with that of traditional method.RESULTS To compare with traditional method,as the golden standard the sensitivity and specificity of PCR method were 98.6% and 100.0%,respectively,the detection could be finished in four hours.Method of PCR was better than traditional method in detecting meticillin-resistant staphylococci.CONCLUSIONS The PCR-based assay is simple,rapid,sensitive and specific,it can be used to detect staphylococci in positive blood culture bottles.
