Studies on the optimum fermentation conditions of marine actinomycete ACMA006 with antitumor activity
- VernacularTitle:抗肿瘤海洋放线菌ACMA006发酵条件的优化
- Author:
Xianwen YUAN
;
Ruili YANG
;
Xue CAO
- Publication Type:Journal Article
- Keywords:
marine actionmycetes;
antitumor substance;
fermentation;
optimization
- From:
Chinese Journal of Marine Drugs
2000;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To increase the yield of antitumor substances from marine actinomycete ACMA006 strain.Methods Fermentation conditions were studied,including combination of medium,the age of strain,ventilatory capacity and cultivation time.Results The results showed that the best medium was as following: soya powder 10g,tryptone 5g,yeast extract paste 10g,soluble starch 15g,glycerol 15g,KH2PO4 0.5g,sea water 1000mL,pH7.2.The best culture conditions were temperature 28℃,the age of the strain 6d,the liquid volume of culture medium 50mL in 150mL Triangular flask,and cultivation time 8d.Conclusion At last 78.1g?L-1 biomass was obtained and the inhibition rate on human liver cancer cells HepG2 was 93.7% when the broth was diluted 4000 times.
