Preparation and study on the physicochemical characteristics of glycoprotein from Crassostrea gigas
- VernacularTitle:牡蛎中糖蛋白成分的分离纯化与理化性质的初步分析
- Author:
Xiaomei FENG
;
Yuqian HAN
;
Zhiqiang ZHAO
;
Huashi GUAN
- Publication Type:Journal Article
- Keywords:
Crassostrea gigas;
glycoprotein;
preparation;
physicochemical characteristics
- From:
Chinese Journal of Marine Drugs
2000;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the preparation and the physicochemical characteristics of glycoprotein from Crassostrea gigas.Methods Oyster collected in Qingdao was taken as the experiment materials,and was extracted with H2O in low temperature.The extracts were purified using Sephacryl s-100 HR gel chromatography and HPLC.Three glycoprotein F22,F33,F42 were finally isolated from the oyster extracts.Results The physicochemical characteristics of glycoprotein were studied.The results showed that the pI value of F22 was 5.5,relative molecular mass of F22 was 34230Da,the protein contents of F22,F33,F42 were 34.1%,19.7% and 12.5%,the saccharide contents of F22,F33,F42 were 35.9%,28.8% and 40%.Conclusion The research result would be used for the study of bioactive component from Crassostrea gigas.
