Screening and isolation of fibrinolytic active compound from marine microorganism
- VernacularTitle:海洋微生物来源纤溶活性化合物的筛选及其分离
- Author:
Yan ZHANG
;
Wenhui WU
;
Peigen ZHOU
;
Bin BAO
;
Zhiwen XIAO
- Publication Type:Journal Article
- Keywords:
marine microorganism;
Czapek medium;
fibrinolysis;
HPLC;
active compound
- From:
Chinese Journal of Marine Drugs
2000;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To isolate bioactive compound of enhancing fibrinolysis from secondary metabolites of marine microorganism.Methods The separation of microorganism from seawater samples,screening of producing fibrinolytic compound's strain,selection of the active strain's optimum fermentation medium and refining of active compound were done by the method of selective cultivation,measuring of compound's fibrinolytic activity and semipreparative HPLC,respectively.Results Nine hundred and thirty-six single strains from 31 samples were collected 100 meters off the coast,and cultures of the fungus(FG216) contained enhancing fibrinolytic compound.Compounds from modified Czapek medium as the fermentation medium of FG216 showed significant fibrinolytic effect.Finally,active fraction were isolated and refined from cultures of FG216.Conclusion In this paper,active compound of enhancing fibrinolysis were gained from secondary metabolites of isolated single microorganism from seawater.
