Protective effects of Haikunshenxi on the kidney and its influence on the expressions of transforming growth factor-?_1 and mRNA in diabetic rats
- VernacularTitle:海昆肾喜对糖尿病肾病大鼠肾脏保护作用及其对肾组织TGF-?_1蛋白及mRNA表达的影响
- Author:
Xinxue ZHANG
;
Zongjiang ZHAO
;
Yang LI
;
Guowen WU
;
Meijuan YANG
- Publication Type:Journal Article
- Keywords:
diabetic nephropathy;
Haikunshenxi;
tansforming growth factor-?1
- From:
Chinese Journal of Marine Drugs
2000;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the expression of tansforming growth factor(TGF)-?1 and mRNA in the renal tubules of Streptozotocin(STZ)-induced diabetic nephropathy rats.Methods SD rats were randomly divided into two groups: normal control and diabetic nephropathy.Diabetes was induced by intraperitoneal injection of STZ.And the diabetic rats were randomly divided into four groups: model group,irbesartan treatment group,Haikunshenxi low and high dose group.Twenty-four-hour urine volume and urinary protein excretion were examined at week 4,8,and 12 respectively.All the rats were sacrificed at week 12,and body weight,kidney weight,and serum excretion,serum creatinine,blood urea nitrogen were examined.The expression of TGF-?1and mRNA in rat renal tissue were detected respectively by immunohistochemistry and in situ hybridization.The graphic analysis system and SPSS 11.5 statistics software were used to treat the results.Results The expression of TGF-?1and mRNA in the Haikunshenxi group was depressed greatly(P
