Effect of heroin on gene expression of PCNA in C6 glioma cells
- VernacularTitle:海洛因对C6细胞增殖细胞核抗原基因表达的影响
- Author:
Xiumei CHI
;
Jizhou ZHANG
;
Mujie KAN
;
Min HONG
- Publication Type:Journal Article
- Keywords:
heroin;
glioma cells;
proliferating cell nuclear antigen;
gene expression
- From:
Journal of Jilin University(Medicine Edition)
2006;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the effect of heroin on the gene expression of proliferating cell nuclear antigen(PCNA) in rat C6 glioma cells,and investigate the molecular mechanism of heroin addiction.Methods The rat C6 glioma cells were treated with heroin with doses of 0-100 mg?L-1 for 24 h,MTT assay was used to detect the viability of cells,the gene expression level of PCNA was determined by RT-PCR.Results When the C6 cells were treated with heroin with doses of 10,20 and 100 mg?L-1,the cell viabilities were 90.62%,80.97%,and 62.73%,there were significant differences compared with control group(P
