Real-time RT-PCR for detection of FOXP3 expression in the peripheral blood mononuclear cells of hepatitis B patients
- VernacularTitle:乙肝患者外周血单个核细胞FOXP3 mRNA表达检测方法的建立及应用
- Author:
Kai GUO
;
Changxing HUANG
- Publication Type:Journal Article
- Keywords:
Hepatitis B;
FOXP3;
Regulatory T cell
- From:
Journal of Medical Postgraduates
2003;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To compare the expression levels of FOXP3 mRNA in the peripheral blood mononuclear cells(PBMC) of hepatitis B patients and healthy persons.Methods: Real-time fluorescence relative quantitative RT-PCR was used to determine the FOXP3 mRNA levels in the PBMCs from 25 patients with chronic hepatitis B and 11 healthy subjects.Results:The FOXP3 mRNA levels were significantly higher in the hepatitis B patients than in the normal subjects(P0.05).Conclusion: The high expression of FOXP3 might be an important factor for the persistence of HBV infection.
