Effect of advanced glycation end products on expression of vascular endothelial growth factor in cultured rabbit retinal M?ller cells
- VernacularTitle:糖基化终产物对视网膜M?ller细胞表达血管内皮生长因子的影响
- Author:
Maoli ZHU
;
Yao LIU
;
Pingsheng CHEN
- Publication Type:Journal Article
- Keywords:
Diabetic retinopathy;
Advanced glycation end products;
Retinal M?ller cells;
Vascular endothelial growth factor
- From:
Chinese Journal of Diabetes
2008;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of advanced glycation end products(AGEs)on expression of vascular endothelial growth factor(VEGF)in rabbit retinal M?ller cells in vitro.Methods We successfully cultured rabbit retinal M?ller cells and made AGEs-BSA as well as its control in vitro.Study with M?ller cells were divided into AGEs-BSA group,AGEs-BSA control group and blank control group.AGEs-BSA group and AGEs-BSA control group were respectively treated with 5 different concentration series of AGEs-BSA and AGEs-BSA control and cultured for 3,6 and 9 days,while blank control group was incubated without any intervention.Then VEGF expression in M?ller cells was observed by immunocytochemistry(ICC).Results Compared with control group,VEGF expression in cultured retinal M?ller cells was significantly enhanced in AGEs-BSA group.And the effects were in the time-and concentration-dependent manners.Conclusions AGEs increases VEGF expression in rabbit retinal M?ller cells in vitro,which indicates that AGEs may promote the progression of diabetic retinopathy(DR)through the induction of VEGF expression.
