Drug Resistance Genes in Imipenem-resistant Pseudomonas aeruginosa
- VernacularTitle:耐亚胺培南铜绿假单胞菌金属?-内酰胺酶基因研究
- Author:
Shuping ZHAO
;
Meijie JIANG
;
Xin TIAN
;
Qiaoguang HAO
- Publication Type:Journal Article
- Keywords:
Pseudomonas aeruginosa;
Imipenem;
Resistance mechanism;
Gene
- From:
Chinese Journal of Nosocomiology
2005;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To study the drug resistance genes in imipenem-resistant Pseudomonas aeruginosa.METHODS Five main drug resistance genes:IMP,VIM,SPM and GIM which are pertinent with metal ?-lactamase and outer membrane protein oprD2 gene were analyzed using PCR method.RESULTS The oprD2 genes in 48 strains of imipenem-resistant P.aeruginosa were negative,and in 14 strains were positive from all 62 strains tested.There were 16 strains with positive IMP type and 5 strains with positive VIM type metal enzyme genes positive,7 metal enzymes producing strains were with negative oprD2 gene.The SPM and GIM metal enzyme genes detected were all negative.CONCLUSIONS The loss of outer membrane protein oprD2 gene is the main mechanism of imipenem resistance in P.aeruginosa in Tai′an area.
